Simultaneous purification and fractionation of nucleic acids and proteins from complex samples using bidirectional isotachophoresis Supporting Information
نویسندگان
چکیده
This document contains the following supplementary figures and information further describing our bidirectional ITP-based technique for simultaneous purification and fractionation of nucleic acids and proteins from complex biological samples: • Figure S-1: Images of on-chip DNA ITP zones and protein ITP zones • Figure S-2: Experimental setup and procedure • Figure S-3: Simulation and visualization of the dynamics of DNA and protein zones in ITP in a straight channel • Figure S-4: Device design for visualization experiments • Figure S-5: Example images of low fluorescence of DNA zones in ITP process
منابع مشابه
Simultaneous purification and fractionation of nucleic acids and proteins from complex samples using bidirectional isotachophoresis.
We report on our efforts to create an on-chip system to simultaneously purify and fractionate nucleic acids and proteins from complex samples using isotachophoresis (ITP). We have developed this technique to simultaneously extract extracellular DNA and proteins from human blood serum samples and deliver these to two separate output reservoirs on a chip. The purified DNA is compatible with quant...
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